CNOT1 antibody

Catalog No. ABIN7112432

The Rabbit Polyclonal anti-CNOT1 antibody has been validated for ELISA, WB, IHC and IP. It is suitable to detect CNOT1 in samples from Human, Mouse and Rat.

Quick Overview for CNOT1 antibody (ABIN7112432)

Target: CNOT1 (CCR4-NOT Transcription Complex, Subunit 1 (CNOT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-CNOT1 Antibody

Purpose: CNOT1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: CCR4-NOT transcription complex, subunit 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CNOT1

Target: CNOT1 (CCR4-NOT Transcription Complex, Subunit 1 (CNOT1))

Alternative Name: CNOT1

Background:

Synonyms: CCR4-NOT transcription complex subunit 1|CCR4-associated factor 1|Negative regulator of transcription subunit 1 homolog (NOT1H, hNOT1)|CNOT1|CDC39|KIAA1007|NOT1

Background: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element(ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem(ES) cell identity.

Molecular Weight: 240-250 kDa, 174 kDa

Gene ID: 23019

UniProt: A5YKK6

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Stem Cell Maintenance